Journal: Materials

Article Title: The Biocompatibility and Self-Healing Effect of a Biopolymer’s Coating on Zn Alloy for Biomedical Applications

doi: 10.3390/ma16237486

Figure Lengend Snippet: Confocal images of U2OS cells with DAPI-stained nucleus after 7 days of incubation on casein-based layer (2cas1h) on tested materials: ZnMg3.2 alloy ( a ); casein coating (2cas1h) ( b ). Additional 3D visualization from Immaris software (Bitplane Scientific Software; version 10.0.0; Olympus, Zurich, Switzerland) for ZnMg3.2 alloy ( c ), casein coating (2cas1h) ( d ).

Article Snippet: Biological characterization was based on a human osteosarcoma cell line U2OS (cat. No. 92022711; Merck, Rahway, NJ, USA).

Techniques: Staining, Incubation, Software

Journal: Cell Death & Disease

Article Title: Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA

doi: 10.1038/s41419-021-04448-3

Figure Lengend Snippet: A The Trio mRNA levels in different cancer type cell lines from the CCLE database. B The expression levels of Trio in sarcoma and normal control tissues were achieved from the TCGA database. C and D Kaplan–Meier curves show the disease-free survival and the overall survival of patients with sarcoma (TCGA database) in terms of Trio gene expression. E and F Kaplan–Meier curves show the disease-free survival and the overall survival of patients with osteosarcoma (TARGET database) in terms of Trio gene expression. G – I The expression levels of Trio in hFOB1.19, U2OS,143B were measured by Western blot and qRT-PCR. J The expression levels of Trio in osteosarcoma and normal control tissues were detected by IHC. Representative images are shown (magnification at ×200). Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01. Scale bars: 100 μm.

Article Snippet: Normal human osteoblast cell line hFOB1.19, as well as human OS cell line U2OS, were purchased from GeneChem (Shanghai, China).

Techniques: Expressing, Control, Gene Expression, Western Blot, Quantitative RT-PCR

Journal: Cell Death & Disease

Article Title: Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA

doi: 10.1038/s41419-021-04448-3

Figure Lengend Snippet: A – C Efficiency of siRNA-mediated Trio knockdown in U2OS and 143B cells was measured by qRT-PCR and Western blot. D SiRNA-mediated Trio knockdown suppressed OS cell growth, as determined by the CCK-8 assay. E Efficiency of shRNA-mediated Trio knockdown in U2OS and 143B cells was detected by GFP. F and G Efficiency of shRNA-mediated Trio knockdown in U2OS and 143B cells was measured by qRT-PCR and Western blot. H and I Trio knockdown suppressed the colony formation of U2OS and 143B cells. J Cell apoptosis was measured by cytometry. Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01. Scale bars: 100 μm.

Article Snippet: Normal human osteoblast cell line hFOB1.19, as well as human OS cell line U2OS, were purchased from GeneChem (Shanghai, China).

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, CCK-8 Assay, shRNA, Cytometry

Journal: Cell Death & Disease

Article Title: Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA

doi: 10.1038/s41419-021-04448-3

Figure Lengend Snippet: A and B Wound-healing assay was used to measure the migration capacity of U2OS and 143B cells. Representative images are shown (magnification at ×100). C and D Transwell migration and Matrigel invasion assays were used to measure the migration and invasion ability of U2OS and 143B cells. Representative images are shown (magnification at ×200). E Cells of the siRNA-Trio group displayed more stress fibers and stable actin structures compared with the control group. Representative images are shown (magnification at ×400). Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01. Scale bars: 100 μm.

Article Snippet: Normal human osteoblast cell line hFOB1.19, as well as human OS cell line U2OS, were purchased from GeneChem (Shanghai, China).

Techniques: Wound Healing Assay, Migration, Control

Journal: Cell Death & Disease

Article Title: Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA

doi: 10.1038/s41419-021-04448-3

Figure Lengend Snippet: A and B EMT markers and Snail were measured by western blotting in U2OS and 143B cells after transfection. C EMT markers and transcription factors (MMP9 and Snail) were measured by qRT-PCR. D U2OS and 143B cells were subjected to immunofluorescence with E-Cadherin and N-Cadherin antibodies. Local enlarged images showed the membrane localization of E-cad. Representative images are shown (magnification at ×200). Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01. Scale bars: 100 μm.

Article Snippet: Normal human osteoblast cell line hFOB1.19, as well as human OS cell line U2OS, were purchased from GeneChem (Shanghai, China).

Techniques: Western Blot, Transfection, Quantitative RT-PCR, Immunofluorescence, Membrane

Journal: Cell Death & Disease

Article Title: Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA

doi: 10.1038/s41419-021-04448-3

Figure Lengend Snippet: A and B osteogenic markers were measured by western blotting in U2OS and 143B cells after transfection. C osteogenic markers and transcription factors were measured by qRT-PCR. D , E U2OS and 143B cells were stained with the ALP kit. Representative images are shown (magnification at ×200). F , G U2OS and 143B cells were stained with ARS solution. Representative images are shown (magnification at 200×). Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01. Scale bars: 100 μm.

Article Snippet: Normal human osteoblast cell line hFOB1.19, as well as human OS cell line U2OS, were purchased from GeneChem (Shanghai, China).

Techniques: Western Blot, Transfection, Quantitative RT-PCR, Staining